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computer assisted analysis  (Olympus)


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    Structured Review

    Olympus computer assisted analysis
    Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
    Computer Assisted Analysis, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 10297 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image+analysis+computer+software/cellSens+Imaging+Software/pmc12939340-97-8-16
    Average 99 stars, based on 10297 article reviews
    computer assisted analysis - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "Stefin B and Cystatin C Deficiency Suppresses Tumor Growth and Alters Tumor Microenvironment in a Breast Cancer Model"

    Article Title: Stefin B and Cystatin C Deficiency Suppresses Tumor Growth and Alters Tumor Microenvironment in a Breast Cancer Model

    Journal: Cells

    doi: 10.3390/cells15040360

    Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
    Figure Legend Snippet: Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

    Techniques Used: Immunodetection, TUNEL Assay

    Related Articles

    Analysis:

    Article Title: Concentration-sensitive control of the stabilization-degradation balance in poly(butylene succinate-co-adipate) films via calcium lignosulfonate and 2,5-dimethyl-2,5-di(tert-butylperoxy)hexane.
    Article Snippet: Balancing durability during use with predictable biodegradation after disposal remains a major challenge in the design of biodegradable polyesters.. Herein, poly(butylene succinate-co-adipate) (PBSA) films were modified with calcium lignosulfonate (LS) and the organic peroxide 2,5-dimethyl-2,5-di(tert-butylperoxy) hexane (DBPH) to elucidate how concentration-dependent intermolecular interactions and radical-mediated processes influence crystallinity, photooxidation, and soil biodegradation.. Solvent-cast films were characterized by ATR-FTIR, DSC, XRD, SEM, AFM, accelerated UV ageing, and ISO 17556 soil biodegradation.

    Article Title: Direct Integration of Electrospun Polycaprolactone Fibers with Polydimethylsiloxane: Influence of Elastomer Formulation on Fiber-Elastomer Adhesion.
    Article Snippet: The resulting stitched composite image was assembled automatically using the FluoView software (cellSens FV v3.2.1.85; Evident Corporation, Tokyo, Japan) to provide a seamless representation of the fiber network and its patterning. .. The image analysis was performed using cellSens Dimension Desktop software (v4.3.1, Olympus Corporation, Tokyo, Japan). .. Patterned fibers in silicone were imaged with a Nikon D7000 digital camera (Nikon, Tokyo, Japan) using Sigma 70 mm F2.8 DG MACRO lens (Sigma, Kawasaki, Japan) and a portable microscope (Sulang Technology, Shenzhen, China).

    Article Title: Orthosteric and allosteric inhibition of the brain V-ATPase outside the alternating-access cycle
    Article Snippet: Focuswas stabilized using the Olympus Zero Drift Correction (ZDC) module. .. Image acquisition and analysis were performed using Olympus cellSens software (v3.2). ..

    Software:

    Article Title: Concentration-sensitive control of the stabilization-degradation balance in poly(butylene succinate-co-adipate) films via calcium lignosulfonate and 2,5-dimethyl-2,5-di(tert-butylperoxy)hexane.
    Article Snippet: Balancing durability during use with predictable biodegradation after disposal remains a major challenge in the design of biodegradable polyesters.. Herein, poly(butylene succinate-co-adipate) (PBSA) films were modified with calcium lignosulfonate (LS) and the organic peroxide 2,5-dimethyl-2,5-di(tert-butylperoxy) hexane (DBPH) to elucidate how concentration-dependent intermolecular interactions and radical-mediated processes influence crystallinity, photooxidation, and soil biodegradation.. Solvent-cast films were characterized by ATR-FTIR, DSC, XRD, SEM, AFM, accelerated UV ageing, and ISO 17556 soil biodegradation.

    Article Title: High genotoxicity of CRISPR/Cas9 versus limited efficacy of CRISPRi in chicken primordial germ cells
    Article Snippet: Fluorescent images were acquired using an Olympus (Evident) SpinSR spinning-disk confocal microscope with appropriate filter sets and a 60× objective. .. All image processing and analysis were performed with Olympus CellSens Dimension software under identical parameters for all groups. .. PGC proliferation and viability under various conditions were assessed using the Cell Counting Kit-8 (CCK-8; Yeasen 40203ES76) colorimetric assay.

    Article Title: B4Galnt1 Deficiency Reverses Severe Neurological Symptoms in a Mouse Model of Tay-Sachs Disease
    Article Snippet: .. Fluorescent images were captured using a BX53F fluorescence microscope (Olympus, Germany), and image analysis was performed with cellSens Entry software (Olympus). ..

    Article Title: Gastric and Small-Intestinal Morphological Remodeling After Intragastric Apelin-13 Administration in Unweaned Rats.
    Article Snippet: .. Microscopic evaluation and image acquisition were performed using the same Olympus BX63 light microscope equipped with a digital camera and cellSens image analysis software (Version 1.5; Olympus, Tokyo, Japan) as described for the histological analysis. ..

    Article Title: Morphological characterization of moulting in the Atlantic horseshoe crab Limulus polyphemus : phylogenetic conservation among chelicerates and evolutionary convergence of ecdysis linked to head shield patterns
    Article Snippet: .. Individuals were observed using an Olympus SZX10 stereomicroscope with an observed magnification of 0.63–6.3× and were imaged using an SC50 5-megapixel colour 118 camera (Olympus Life Science Solutions, Olympus, Tokyo, Japan) with Preciv Image Analysis Software (version 1.2; Evident Corporation, Tokyo, Japan). ..

    Article Title: Direct Integration of Electrospun Polycaprolactone Fibers with Polydimethylsiloxane: Influence of Elastomer Formulation on Fiber-Elastomer Adhesion.
    Article Snippet: The resulting stitched composite image was assembled automatically using the FluoView software (cellSens FV v3.2.1.85; Evident Corporation, Tokyo, Japan) to provide a seamless representation of the fiber network and its patterning. .. The image analysis was performed using cellSens Dimension Desktop software (v4.3.1, Olympus Corporation, Tokyo, Japan). .. Patterned fibers in silicone were imaged with a Nikon D7000 digital camera (Nikon, Tokyo, Japan) using Sigma 70 mm F2.8 DG MACRO lens (Sigma, Kawasaki, Japan) and a portable microscope (Sulang Technology, Shenzhen, China).

    Article Title: Orthosteric and allosteric inhibition of the brain V-ATPase outside the alternating-access cycle
    Article Snippet: Focuswas stabilized using the Olympus Zero Drift Correction (ZDC) module. .. Image acquisition and analysis were performed using Olympus cellSens software (v3.2). ..

    Article Title: B4Galnt1 Deficiency Reverses Severe Neurological Symptoms in a Mouse Model of Tay-Sachs Disease.
    Article Snippet: .. Fluorescent images were captured using a BX53F fluorescence microscope (Olympus, Germany), and image analysis was performed with cellSens Entry software (Olympus). ..

    Fluorescence:

    Article Title: B4Galnt1 Deficiency Reverses Severe Neurological Symptoms in a Mouse Model of Tay-Sachs Disease
    Article Snippet: .. Fluorescent images were captured using a BX53F fluorescence microscope (Olympus, Germany), and image analysis was performed with cellSens Entry software (Olympus). ..

    Article Title: B4Galnt1 Deficiency Reverses Severe Neurological Symptoms in a Mouse Model of Tay-Sachs Disease.
    Article Snippet: .. Fluorescent images were captured using a BX53F fluorescence microscope (Olympus, Germany), and image analysis was performed with cellSens Entry software (Olympus). ..

    Microscopy:

    Article Title: B4Galnt1 Deficiency Reverses Severe Neurological Symptoms in a Mouse Model of Tay-Sachs Disease
    Article Snippet: .. Fluorescent images were captured using a BX53F fluorescence microscope (Olympus, Germany), and image analysis was performed with cellSens Entry software (Olympus). ..

    Article Title: B4Galnt1 Deficiency Reverses Severe Neurological Symptoms in a Mouse Model of Tay-Sachs Disease.
    Article Snippet: .. Fluorescent images were captured using a BX53F fluorescence microscope (Olympus, Germany), and image analysis was performed with cellSens Entry software (Olympus). ..

    Light Microscopy:

    Article Title: Gastric and Small-Intestinal Morphological Remodeling After Intragastric Apelin-13 Administration in Unweaned Rats.
    Article Snippet: .. Microscopic evaluation and image acquisition were performed using the same Olympus BX63 light microscope equipped with a digital camera and cellSens image analysis software (Version 1.5; Olympus, Tokyo, Japan) as described for the histological analysis. ..



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    Image Search Results


    Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

    Journal: Cells

    Article Title: Stefin B and Cystatin C Deficiency Suppresses Tumor Growth and Alters Tumor Microenvironment in a Breast Cancer Model

    doi: 10.3390/cells15040360

    Figure Lengend Snippet: Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

    Article Snippet: The average size of metastases was quantified using computer-assisted analysis with CellSens Imaging software (Version 1.17; Olympus, Tokyo, Japan).

    Techniques: Immunodetection, TUNEL Assay